Journal: Iranian Journal of Basic Medical Sciences

Article Title: Anti-inflammatory and anti-apoptotic effects of hyperbaric oxygen preconditioning in a rat model of cisplatin-induced peripheral neuropathy

doi: 10.22038/IJBMS.2019.40095.9504

Figure Lengend Snippet: Light photomicrographs show immunohistochemical expression of TNF-α in the sciatic nerve of sham (7A), HBO (7B), control (7C), and precondition (7D) groups. The positive staining of TNF-α is presented by brown color of cytoplasm (arrows) (scale bar = 100 µm). Densitometry analysis of immunohistochemical photomicrographs for TNF-α was assessed in all experimental groups using the ImageJ software package (7E). Data are expressed as a percentage of total tissue area. ***P<0.001 vs sham and HBO groups; ###P<0.001 vs control group

Article Snippet: The sections were incubated in anti-caspase 3 rabbit polyclonal antibody (1:50 in PBS, v/v, Abcam), anti-TNF-α rabbit polyclonal antibody (1:50 in PBS, v/v, Elabscience), or anti-iNOS rabbit polyclonal antibody (1:50 in PBS, v/v, Abcam) at 4 ° C for 24 hr.

Techniques: Immunohistochemical staining, Expressing, Control, Staining, Software

Journal: Regenerative Biomaterials

Article Title: Triamcinolone acetonide-loaded chitosan-polyethylene glycol hydrogel for preventing esophageal stricture post-endoscopic submucosal dissection

doi: 10.1093/rb/rbag002

Figure Lengend Snippet: Schematic diagram of QCS-PEG@TA hydrogel preventing stricture after ESD operation. ( A ) The hydrogel is synthesized via gelation of quaternized chitosan (QCS) and 8-arm polyethylene glycol (8-arm PEG) with the addition of triamcinolone acetonide (TA), as depicted by molecular diagrams. ( B ) Upon endoscopic injection into the submucosal layer of a porcine esophagus, the hydrogel forms a biocompatible barrier, releasing therapeutic agents locally. ( C ) Anti-inflammatory effects are demonstrated by increased M2 macrophage activity and reduced TNF-α/β levels, while anti-fibrotic action is evidenced by decreased collagen I deposition and α-SMA secretion. Such a dual-pathway regulation addresses post-ESD stenosis, providing a translational approach for the treatment and management of esophageal stricture in clinical settings.

Article Snippet: Afterwards, the slices were incubated with IL-1β (Cat No. AF7209, Beyotime), TNF-α (Cat No. bs-2150R, Bioss), TNF-β (Cat No. bs-0093R, Bioss), Cytokeratin 5 (Cat No. bs-1060R, Bioss), CD31 (Cat No. bs-0195R, Bioss), Collagen I (Cat No. bs-0578R, Bioss), α-SMA (Cat No. bsm-33187M, Bioss) overnight at 4°C, respectively.

Techniques: Synthesized, Injection, Activity Assay

Journal: Regenerative Biomaterials

Article Title: Triamcinolone acetonide-loaded chitosan-polyethylene glycol hydrogel for preventing esophageal stricture post-endoscopic submucosal dissection

doi: 10.1093/rb/rbag002

Figure Lengend Snippet: Cytotoxicity, macrophage polarization and cell migration of hydrogel. The cytotoxicity was evaluated by ( A ) live/dead staining on the first, second and third day after processing with different materials and ( B ) CCK-8 assay ( n = 5). ( C ) Flow cytometry analysis of F4/80 and CD206 expression. ( D ) Percentage of M2 macrophages (F4/80 + CD206 + , n = 3). ( E ) TNF-α and ( F ) TGF-β in cell supernatant after different treatments detected by ELISA ( n = 3). ( G ) Cell migration assay and ( H ) migration ratio of L929 cells incubation with different treatments for 48 h ( n = 3). * P < 0.05, ** P < 0.01 and *** P < 0.001.

Article Snippet: Afterwards, the slices were incubated with IL-1β (Cat No. AF7209, Beyotime), TNF-α (Cat No. bs-2150R, Bioss), TNF-β (Cat No. bs-0093R, Bioss), Cytokeratin 5 (Cat No. bs-1060R, Bioss), CD31 (Cat No. bs-0195R, Bioss), Collagen I (Cat No. bs-0578R, Bioss), α-SMA (Cat No. bsm-33187M, Bioss) overnight at 4°C, respectively.

Techniques: Migration, Staining, CCK-8 Assay, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Cell Migration Assay, Incubation

Journal: Regenerative Biomaterials

Article Title: Triamcinolone acetonide-loaded chitosan-polyethylene glycol hydrogel for preventing esophageal stricture post-endoscopic submucosal dissection

doi: 10.1093/rb/rbag002

Figure Lengend Snippet: QCS-PEG@TA Hydrogel reduced the expression of inflammatory factors, angiogenesis and collagen deposition. Immunohistochemistry staining ( A ) and quantitative statistics ( B ) of TNF-α, TNF-β, CD31, α-SMA, collagen-I and CK5 on Day 7 postsurgery (scale bar, 100 μm; n = 3). Immunohistochemistry ( C ) and quantitative statistics ( D ) of TNF-α, TNF-β, CD31, α-SMA, collagen-I and CK5 on Day 28 postsurgery (scale bar, 1 mm; n = 3). * P < 0.05 and ** P < 0.01.

Article Snippet: Afterwards, the slices were incubated with IL-1β (Cat No. AF7209, Beyotime), TNF-α (Cat No. bs-2150R, Bioss), TNF-β (Cat No. bs-0093R, Bioss), Cytokeratin 5 (Cat No. bs-1060R, Bioss), CD31 (Cat No. bs-0195R, Bioss), Collagen I (Cat No. bs-0578R, Bioss), α-SMA (Cat No. bsm-33187M, Bioss) overnight at 4°C, respectively.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Clinical science (London, England : 1979)

Article Title: Sodium bicarbonate loading limits tubular cast formation independent of glomerular injury and proteinuria in Dahl salt-sensitive rats

doi: 10.1042/CS20171630

Figure Lengend Snippet: Reagents/antibodies

Article Snippet: TNF α -biotin , Bioss , bs-2081R-biotin , AE081731 , 100 μ1 , rabbit.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Inflammation-induced TRPV4 channels exacerbate blood–brain barrier dysfunction in multiple sclerosis

doi: 10.1186/s12974-024-03069-9

Figure Lengend Snippet: Primary antibody details

Article Snippet: TNFα , Rabbit , 1:200 , Acetone , Origene , PP1071P1 , IHC FF.

Techniques: Plasmid Preparation