Journal: Frontiers in Cellular Neuroscience

Article Title: A Novel Hydrogen Sulfide Donor Reduces Pilocarpine-Induced Status Epilepticus and Regulates Microglial Inflammatory Profile

doi: 10.3389/fncel.2021.780447

Figure Lengend Snippet: The H 2 S donor downregulated the levels of microglial pro-inflammatory profile in vivo . (A,B) Representative images of iNOS (red) with Iba1 (green) immunostaining or (C,D) Arg1 (red) with Iba1 (green) immunostaining in the CA1 and CA3 regions of the hippocampus in control and the H 2 S donor-treated SE mice. Scale bar 25 μm in the merged panel and 5 μm in the magnified panel. Magnified images are expansions of boxed areas in corresponding panels in the left of each magnified image. (E) Quantitative analysis of Iba1 + cells in CA1 and CA3 regions of the hippocampus. (F) Quantitative data are shown the ratio of iNOS + : Iba1 + to total Iba1 + cells. n = 6 per group. *** p < 0.001 vs SE. Two-way ANOVA with Bonferroni post hoc tests. (G) Quantitative data are shown as the ratio of Arg1 + : Iba1 + to total Iba1 + cells. n = 6 per group. *** p < 0.001 vs SE. Student’s t test. (H–K) Western blot assay shows that expression levels of microglial pro-inflammatory markers (COX2 and TNF-α) are increased in the hippocampus of SE mice. However, the H 2 S donor decreased COX2 and TNF-α expression and increased Arg-1and IL-10 expression in SE mice. The results are expressed as the mean±SEM. n = 3 per group.* p < 0.05, ** p < 0.01 vs Control, # p < 0.05, ## p < 0.01 vs SE. One-way ANOVA with Bonferroni post hoc tests.

Article Snippet: Then, the membranes were blocked with bovine serum albumin (BSA), and incubated with rabbit anti-COX2 (1:500, #12375-1-AP, Proteintech Group, United States), rabbit anti-Arg-1 (1:4,000, #16001-1-AP, Proteintech Group, United States), rabbit anti-TNF-α (1:1,000, #bs-0078R, BIOSS, China), rabbit anti-IL-10 (1:1,000, #bs-20373R, BIOSS, China), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:8,000, #60004-1-Ig, Proteintech Group, United States), and rabbit anti-Tubulin (1:1,000, #11224-1-AP, Proteintech Group, United States) at 4°C overnight.

Techniques: In Vivo, Immunostaining, Western Blot, Expressing

Journal: Frontiers in Cellular Neuroscience

Article Title: A Novel Hydrogen Sulfide Donor Reduces Pilocarpine-Induced Status Epilepticus and Regulates Microglial Inflammatory Profile

doi: 10.3389/fncel.2021.780447

Figure Lengend Snippet: The H 2 S donor downregulated the levels of microglial pro-inflammatory profile in vitro . (A–C,E) Immunofluorescent staining and quantification of iNOS and Arg1 in lipopolysaccharide (LPS)-treated BV2 cells. Scale bars, 50 μm for the original images and 10 μm for the magnified images. n = 6 per group. *** p < 0.001 vs Control, # p < 0.05, ### p <0.001 vs LPS. Statistical significance was determined by two-way ANOVA with Bonferroni post hoc tests. (D,F) Expression levels of microglial pro-inflammatory markers (COX2, TNF-α) and microglial anti-inflammatory markers (Arg1, IL-10) in BV2 cells were determined by Western blotting. The H 2 S donor decreased the expression of pro-inflammatory markers and upregulated the expression of anti-inflammatory markers in LPS-induced inflammation model of microglia. n = 3 per group. * p < 0.05, ** p < 0.01 vs Control, # p < 0.05, ## p < 0.01 vs LPS. One-way ANOVA with Bonferroni post hoc tests.

Article Snippet: Then, the membranes were blocked with bovine serum albumin (BSA), and incubated with rabbit anti-COX2 (1:500, #12375-1-AP, Proteintech Group, United States), rabbit anti-Arg-1 (1:4,000, #16001-1-AP, Proteintech Group, United States), rabbit anti-TNF-α (1:1,000, #bs-0078R, BIOSS, China), rabbit anti-IL-10 (1:1,000, #bs-20373R, BIOSS, China), mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:8,000, #60004-1-Ig, Proteintech Group, United States), and rabbit anti-Tubulin (1:1,000, #11224-1-AP, Proteintech Group, United States) at 4°C overnight.

Techniques: In Vitro, Staining, Expressing, Western Blot

Journal: Clinical science (London, England : 1979)

Article Title: Sodium bicarbonate loading limits tubular cast formation independent of glomerular injury and proteinuria in Dahl salt-sensitive rats

doi: 10.1042/CS20171630

Figure Lengend Snippet: Reagents/antibodies

Article Snippet: TNF α -biotin , Bioss , bs-2081R-biotin , AE081731 , 100 μ1 , rabbit.

Techniques:

Journal: Journal of Neuroinflammation

Article Title: Inflammation-induced TRPV4 channels exacerbate blood–brain barrier dysfunction in multiple sclerosis

doi: 10.1186/s12974-024-03069-9

Figure Lengend Snippet: Primary antibody details

Article Snippet: TNFα , Rabbit , 1:200 , Acetone , Origene , PP1071P1 , IHC FF.

Techniques: Plasmid Preparation